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Starbase 227 was a Federation Starfleet starbase active in the 24th century.

Admiral Chekote commanded Starbase 227 in 2370. He ordered the USS Enterprise-D to investigate Captain Jean-Luc Picard's "murder". (TNG: "Gambit, Part I")

In 2374, renegade geneticist Dr. Alan Murrow was being held by Starfleet on Starbase 227, where he was liberated by Khan Noonien Singh. (USS Baldwin: "The Return of Khan")

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